It is well known the important function of after-sun products in relieving the effects resulting from sun exposure and in improving the skin conditions by increasing the hydration, through a soothing, refreshing and restructuring action. In order to be able to evaluate in vitro the efficacy of that specific typology of cosmetic products, Bio Basic Lab has developed a new protocol, structured on three different levels. This allows demonstrating the repairing action against the damages caused by UV radiations, both in terms of improvement of cell conditions and of repair against the oxidative damages, and demonstrating the soothing action. The test is performed on keratinocytes cell coltures and it is based on the following evaluations: -Vitality evaluation and possible total protein dosage after different times of application of the product, following UV radiation. - Evaluation of the oxidative damage reduction, derived by the exposure to UV radiation, through a dosage of free radicals, produced by those cells treated with the product under test. - Evaluation of the soothing action through an analysis of IL 1α synthesis in those cells treated with the cosmetic product under test, after exposure to UV radiations. Thanking you for your attention, we remind that we are always available to provide more information and to develop the most suitable experimental protocols for meeting your specific requirements, and to your different cosmetic formulations. BIO BASIC EUROPE In Vitro Tests Responsible Dr.ssa Eliana Regola +39024155729 testing@biobasiceurope.it BIO BASIC EUROPE Technical Director Dr. Claudio Angelinetta BIO BASIC EUROPE Managing Director Dr. Umberto Pianca