Before being distributed on the global market, products, according to the regulations of their sector, shall be tested to be safe for human health, if used under normal or reasonably foreseeable conditions. In particular, the European Regulation n. 1223/2009 bans animal testing on finished cosmetic products and ingredients (or combinations of ingredients). This restriction has favoured the development of alternative in vitro methods, to protect companies and consumers, and that, unlike the animal model, allow the experiment to be carried out without numerical limits, and under conditions of greater control and standardization, as well as to apply quantitative and objective measures. After having performed the toxicological evaluation of the product, it is possible to check its safety through in vitro assays on cell cultures and reconstructed human tissues. These tests are carried out by professionals at BIO BASIC LAB, other specialized laboratories and university institutes.