EVALUATION OF THE ABILITY TO INCREASE THE SAMPLE ION OF SPECIFIC MARKER (COLLAGEN, ELASTIN, HYALURONIC ACID ...)
Aim of the test is to evaluate the ability of a cosmetic sample to modulate the synthesis of specific markers (collagen, elastin, hyaluronic acid ...) in cultures of keratinocytes / fibroblasts treated with the sample to be tested.
IN VITRO EVALUATION OF ANTIOXYDANT ACTIVITY
Aim of test is to assess if the tested sample, at different concentrations, is able to prevent the sample ion of reactive oxygen species (ROS) (ANTIOXIDANT ACTIVITY)
IN VITRO EVALUATION OF SCAVENGER ACTIVITY
Aim of the test is to evaluate if the tested sample, at different concentrations, is able to neutralize the reactive oxygen species (ROS free radicals).
IN VITRO EVALUATION OF THE REGENERATING ACTIVITY
Aim of the test is to assess the ability of a cosmetic sample to stimulate cell regeneration of fibroblasts/keratinocytes or other cellular lines in vitro.
EVALUATION OF THE INHIBITION OF HYALURONIDASE
The purpose of the test is to evaluate the ability of the sample under examination to inhibit the activity of the enzyme hyaluronidase. Inhibition of hyaluronidase activity prevents rapid degradation of hyaluronic acid and is essential for maintaining the structural integrity of the cell membrane. Any enzyme inhibitors, by maintaining hyaluronic acid homeostasis, could act as anti-aging factors.
EVALUATION OF PROTECTIVE ACTION
The purpose of the tests is to evaluate the ability of the sample under examination to protect cell cultures from stress caused by the activation of MMP-1.
IN VITRO EVALUATION OF LIPOREDUCING ACTIVITY
The purpose of the test is to determine the liporeductive activity of a sample both through volumetric assessment of adipocytes (morphological analysis) and by measuring the variation in triglyceride accumulation in adipocytes.
IN VITRO EVALUATION OF VOLUMIZING ACTIVITY
The purpose of the test is to determine the volumizing activity of a sample both through volumetric assessment and by measuring the variation in triglyceride accumulation in adipocytes.
IN VITRO EVALUATION OF MUCOADESIVITY
The test is performed on cell cultures of the mucosa or on reconstructed human mucosal models to evaluate the in vitro mucoadhesiveness of the tested sample. Mucoadhesiveness is determined by assessing the percentage of inhibition of the lectin-glycoprotein binding in mucosal cells or on reconstructed human mucosal models in vitro.
ABSORPTION TEST AND ASSESSMENT OF THE EFFICACY OF SPECIFIC MARKERS
The purpose of the test is to evaluate, in vitro, the absorption of specific markers contained in a sample through reconstructed human tissues and, if necessary, to verify the maintenance of the specific efficacy of the markers after absorption through in vitro efficacy testing on cell cultures.
ABSORPTION TEST CELLULAR MONOSTRATE OF INTESTINAL CELLS AND ASSESSMENT OF THE EFFICACY OF SPECIFIC MARKERS
The purpose of the test is to evaluate, in vitro, the absorption of specific markers contained in a sample through a monolayer of intestinal cells (Caco-2) and, if necessary, to verify the maintenance of the markers' efficacy after absorption through in vitro efficacy testing on cell cultures.
EVALUATION OF THE PERCUTANEOUS ABSORPTION (OECD 428)
Aim of the test is to define the kinetics of dermal permeation (stratum corneum, vital epidermis and dermis) and transdermal of active substances through synthetic membranes or isolated skin by the use of the Franz cell.